Banca de QUALIFICAÇÃO: Diogo de Amorim Barros

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : Diogo de Amorim Barros
DATA : 12/03/2024
HORA: 14:30
LOCAL: Auditório 1
TÍTULO:

AVALIAÇÃO IN VITRO DA ATIVIDADE ANTIFÚNGICA DE CÁPULAS VAGINAIS CONTENDO EXTRATOS LIOFILIZADOS DE ESPÉCIES DE PLANTAS DO CERRADO E LACTOBACILLUS SPP. EM CEPAS ATCC DE CANDIDA SPP

PALAVRAS-CHAVES:

Lactobacillus spp., Candida spp., Vulvovaginal candidiasis, vaginal capsule, plant extract.

PÁGINAS: 100
RESUMO:

Vulvovaginal Candidiasis (VVC) represents the second largest cause of vaginal infections and is diagnosed at least once in a lifetime in 75% of women, and 40-50% of these present a second episode over the years. Around 85% of vulvovaginal candidiasis is caused by strains of Candida albicans and the rest by non-albicans species, with 5 to 15% of these infections being caused by Candida glabrata. The main symptoms associated with vulvovaginal candidiasis are intense vulvar itching, burning, leucorrhoea, dyspareunia, dysuria, edema, vulvovaginal erythema, vulvovaginal fissures, and white, odorless, thick, irregular vaginal discharge. At the same time, in the vaginal microbiota, Lactobacillus spp. are the largest number of microorganisms and are commensal bacteria that help control strains of Candida spp. With this information and knowing that some plant extracts, through the chemical variability of their secondary metabolites, may also present antifungal activity against species of Candida spp., the present work aimed to evaluate the microbicidal activity of plant extracts from the Cerrado, as well as the association with Lactobacillus rhamnosus and Lactobacillus crispatus against ATCC strains of Candida albicans 90028, Candida glabrata 90030 and Candida krusei 6258. For this, a broth microdilution test was carried out to determine the Minimum Inhibitory Concentration (MIC) of the extracts and in vitro assays of vaginal capsules containing extracts from selected Cerrado’s plants and Lactobacillus spp. Morus nigra and Pouteria torta extract were analyzed alone at concentrations of 25 mg, 50 mg and 100 mg, or in association with Lactobacillus crispatus 1.0 x 109 UFC, or with Lactobacillus rhamnosus at the same concentration, in YM and MRS broth. The plants species with the greatest potential for inhibiting the growth of Candida albicans were the aqueous extract of Miconia chamissois and Pouteria ramiflora, MIC 3.125 mg/mL. As for Candida glabrata, the species that showed the greatest inhibition potential were aqueous extract of Miconia chamissois and aqueous extract of Psidium guajava with MIC equal to 3.125 mg/mL and 6.25 mg/mL, respectively. Finally, for Candida krusei, the plants species with the lowest inhibition concentration were Miconia chamissois MIC 3.125 mg/mL, Psidium guajava and Pouteria torta with MIC 6.25mg/mL, however only Pouteria torta, among these presented pharmacotechnical viability for presentation as vaginal capsules. Co-cultivation with L. crispatus and L. rhamnosus enhanced the microbicidal action of the extracts on Candida spp. evaluated. Morus nigra extract showed microbicidal action of 89% against C. albicans, 98% against C. glabrata and 90% against C. krusei. The Pouteria torta extract presented 93%, 98% and 90% respectively on the same Candida species evaluated. It can also be inferred that depending on the species of Candida tested, spp. the species of L.crispatus and L. rhamnosus reduce in number after co-cultivation, but increase the microbicidal action, pointing to the existence of a synergism between the extract and Lactobacillus spp. against the evaluated yeasts. Although these preliminary data point to the need for additional studies, they indicate an important combined action between plant extracts and Lactobacillus spp. as a treatment strategy or therapeutic adjunct for CVV, especially with non-albicans species such as C. glabrata and C. krusei, which are often resistant to available treatments.

MEMBROS DA BANCA:
Interno - 1880131 - GUILHERME MARTINS GELFUSO
Presidente - 1562111 - PEROLA DE OLIVEIRA MAGALHAES DIAS BATISTA
Externa ao Programa - 2376979 - YANNA KARLA DE MEDEIROS NOBREGA - UnBInterna - 1844214 - YRIS MARIA FONSECA BAZZO